Study gut microbiome 16S rRNA gene versus gut microbiota in HCV patients

El-Badawy, Reda; El Husseini, Naglaa; ElShewi, Mohamed; El Azab, Tamer; Mohammmedy, Ehrahim; Sayed, Ahmed Mohamed

doi:10.21608/ijisd.2020.73511

Study gut microbiome 16S rRNA gene versus gut microbiota in HCV patients

Document Type : Original Article

Authors

¹ Departments of Hepatology, Gastroenterology and Infectious Diseases, Benha University.

² Microbiology and Immunology, Medical Biochemistry, Benha University.

³ Departments of Hepatology, Gastroenterology and Infectious Diseases, Benha University

⁴ Departments of Hepatology, Gastroenterology and Infectious Diseases Benha University.

⁵ Hepatology, Gastroenterology and Infectious Diseases Department, Dar El Sheifa Hospital

⁶ Boulak El Dakror general hospital, Giza, Egypt.

10.21608/ijisd.2020.73511

Abstract

Background &Objectives: Still there is percentage of HCV patients not responding to Direct Acting Antiviral Agents (DAAS). Liver can be greatly affected by changes in gut Microbiome, microbiota. The study was done to evaluate the association between gut microbiome versus microbiota in HCV patients and the response to DAAS .Subjects and Methods: Thirty HCV patients aged from20-60years old ,both males and femals; group 1 (No=15 of HCV responders and group 2 (No=15 relapser HCV patients) treated by DAAS according to the treatment protocol of the Egyptian National Committee for Control of Viral Hepatitis (NCCVH).Group 3 healthy control subjects (No=15) . Full history taking, clinical examination and all investigations were done plus stool culture by Vitek 2and 16srRNA gene amplification and sequencing were done as per Clinilab Maadi ,Cairo .Statistical analysis were done after tabulation of the results ,p<0.05 considered significant. Results: 16SrRNA gene microbiome was of statistically significant decrease in (6) micrbiota in non-responders and increased in (2) .Microbiota compared to responder group ( with P value: ranged from 0.06 to 0.013) Microbiota which increased in non-responder group than responder group ( with P value: ranged from 0.002 to 0.003).The number of microbiota by culture is completely defected and different between the groups compared to 16SrRNA gene for microbiome.. Conclusion: Microbiome more accurate and specific than stool culture for detection of microbiota

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